ppat.1009431.s001.pdf (875.9 kB)
Details of HT-SGS process from sample to sequencing.
journal contribution
posted on 2021-04-08, 17:27 authored by Sung Hee Ko, Elham Bayat Mokhtari, Prakriti Mudvari, Sydney Stein, Christopher D. Stringham, Danielle Wagner, Sabrina Ramelli, Marcos J. Ramos-Benitez, Jeffrey R. Strich, Richard T. Davey Jr., Tongqing Zhou, John Misasi, Peter D. Kwong, Daniel S. Chertow, Nancy J. Sullivan, Eli A. BoritzSARS-CoV-2 genomic RNA (gRNA) is reverse-transcribed with a primer that binds in ORF6, downstream of the M gene stop codon, and includes a UMI sequence of 8 random nucleotides flanked by a PCR reverse primer binding site. Reverse-transcription products are amplified by PCR using a forward primer that binds in ORF1, upstream of the spike gene start codon. Amplified products are then subjected to long-read sequencing.
(PDF)
History
Usage metrics
Categories
Keywords
WAhumoral immunityCOVID -19 Tracking evolutionspike NH 2COVID -19. SARS-CoV -2 genomessyndrome coronavirus 2furin cleavage site regionsvirus RNA genomes7 study participantsmutationSARS-CoV -2 exhibitselucidate coronavirus disease 2019spike NTD epitopeSARS-CoV -2 spike variantssampleSARS-CoV -2 virion surface proteinsSARS-CoV -2 passaged4 virus haplotypes
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC