ppat.1010138.s004.docx (394.71 kB)
Deletion of TgGRA14 in type II strain.
journal contribution
posted on 2021-12-13, 18:36 authored by Yolanda Rivera-Cuevas, Joshua Mayoral, Manlio Di Cristina, Anna-Lisa E. Lawrence, Einar B. Olafsson, Romir K. Patel, Dishari Thornhill, Benjamin S. Waldman, Akira Ono, Jonathan Z. Sexton, Sebastian Lourido, Louis M. Weiss, Vern B. CarruthersA. Schematic for the deletion of the HXGPRT gene with primer binding sites B. PCR to validate the deletion of HXGPRT. C. Schematic for the deletion of the TgGRA14 with primer amplification sites. D. PCR to validate the deletion of TgGRA14 in the ME49Δku80 background. E. Immunoblot confirming the deletion of TgGRA14 in the MΔgra14 strain.
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master manipulator capablelate domain motifsfusogenic parasitophorous vacuolecontains motifs homologousparasite proteins includinghost cytosolic proteinsdense granule proteinsdeficient parasites revealedhost escrt componentsparasite gains resourcestoxoplasma gondii dependent vlp buddinghost escrt machineryparasite uptakehost cytosolhost cellwt parasitesvesicular buddinggondii dense granulesmediate escrtresiding withinreplicative nicherelease assaypvm ).molecular underpinningsmarked reductionlimiting membranelike particlekey pathwayintracellular subsistenceeffectively siphoningcontaining portionalso showaccesses contents1 virus1 gag
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