DRG filtering as recorded in isoflurane anesthetized rats.
The recordings similar to that shown in Fig 1 but performed in male rats anesthetized with isoflurane. (A) After baseline was recorded (control), Capsaicin (CAP, 10 μM, 50 μl) was injected into the hindpaw. Application of GABA (200 μM, 3 μl) to DRG reduced CAP-induced firing frequency in DR but not SN (bottom traces). (B) Summary for panel A. Two-factor (nerve site, drug application) repeated measures ANOVA: main effects associated with nerve site [F(1,20) = 25.5; p < 0.001] and drug application [F(2,19) = 11.8; p < 0.01]; significant interaction between nerve site and drug application [F(2,19) = 9.4; p < 0.01]. Bonferroni post hoc test: **,***significant difference from control (p < 0.01, p < 0.001); #significant difference from CAP (p < 0.05). (C) GABAA antagonist bicuculline (BIC, 200 μM, 3 μl) was applied to DRG; hindpaw was not stimulated. (D) Summary for panel C. Two-factor repeated measures ANOVA: main effects associated with nerve site [F(1,22) = 17.4; p < 0.01], drug application [F(1,22) = 21.4; p < 0.001], significant interaction between nerve site and drug application [F(1,22) = 13.6; p < 0.01]. Bonferroni post hoc test: ***significant difference from control (p < 0.001). Metadata for quantifications presented in this figure can be found at https://archive.researchdata.leeds.ac.uk/1042/.
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