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Cross-Linking Chemistry of Tyramine-Modified Hyaluronan Hydrogels Alters Mesenchymal Stem Cell Early Attachment and Behavior
journal contribution
posted on 2017-02-01, 00:00 authored by Claudia Loebel, Spencer E. Szczesny, Brian D. Cosgrove, Mauro Alini, Marcy Zenobi-Wong, Robert L. Mauck, David EglinGiven the significance of hydrogels
as cell-instructive materials,
it is important to understand how differences in their chemical and
physical properties are able to direct cell fate. For example, it
remains unclear how different hydrogel cross-linking chemistries and
gelation mechanisms influence cell behavior. Here, we report on hyaluronan-tyramine
(HA-Tyr) hydrogels prepared either with enzymatic cross-linking using
horseradish peroxidase and H2O2 or with visible
light (500 nm) triggered gelation. We demonstrate that when hydrogels
are polymerized to equivalent Young's moduli, the specific cross-linking
chemistry of HA-Tyr hydrogels can have a substantial impact on mesenchymal
stem cell (MSC) behavior. MSCs cultured on HA-Tyr hydrogels exhibit
increased cell spread areas on enzymatically formed substrates relative
to photo-cross-linked matrices. While enzymatically formed hydrogels
led to MSCs exhibiting greater cell focal adhesion length, MSCs cultured
on the photo-cross-linked matrices exhibited smaller cell spread area
and shorter focal adhesion length but generated increased traction
stress. These findings highlight the importance of understanding hydrogel
cross-linking chemistries when the role of biophysical cues in regulating
stem cell fate is investigated.
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understanding hydrogel cross-linking chemistriescell fateMSCadhesion lengthcell spread areaHA-Tyr hydrogels exhibitphoto-cross-linked matriceshydrogel cross-linking chemistriesTyramine-Modified Hyaluronan Hydrogels Alters Mesenchymal Stem Cellcell spread areasgelation mechanisms influence cell behaviorH 2 O 2
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