am7b13929_si_001.pdf (292.88 kB)
Core–Shell Silver Nanoparticles in Endodontic Disinfection Solutions Enable Long-Term Antimicrobial Effect on Oral Biofilms
journal contribution
posted on 2017-09-18, 00:00 authored by Elif Ertem, Beatrice Gutt, Flavia Zuber, Sergio Allegri, Benjamin Le Ouay, Selma Mefti, Kitty Formentin, Francesco Stellacci, Qun RenTo
achieve effective long-term disinfection of the root canals, we synthesized
core–shell silver nanoparticles (AgNPs@SiO2) and
used them to develop two irrigation solutions containing sodium phytate
(SP) and ethylene glycol-bis(β-aminoethyl ether)N,N,N′,N′-tetraacetic acid (EGTA), respectively. Ex vivo studies with instrumented root canals revealed that the developed
irrigation solutions can effectively remove the smear layer from the
dentinal surfaces. Further in vitro experiments with
single- and multispecies biofilms demonstrated for the first time
that AgNPs@SiO2-based irrigation solutions possess excellent
antimicrobial activities for at least 7 days, whereas the bare AgNPs
lose the activity almost immediately and do not show any antibacterial
activity after 2 days. The long-term antimicrobial activity exhibited
by AgNPs@SiO2 solutions can be attributed to the sustainable
availability of soluble silver, even after 7 days. Both solutions
showed lower cytotoxicity toward human gingival fibroblasts compared
to the conventionally used solution (3% NaOCl and 17% EDTA). Irrigation
solutions containing AgNP@SiO2 may therefore be highly
promising for applications needing a long-term antimicrobial effect.