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Conditions used for cryoET data collection and processing of WT and mutant NEC coats in this study.

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posted on 2024-01-16, 18:30 authored by Elizabeth B. Draganova, Hui Wang, Melanie Wu, Shiqing Liao, Amber Vu, Gonzalo L. Gonzalez-Del Pino, Z. Hong Zhou, Richard J. Roller, Ekaterina E. Heldwein

Conditions used for cryoET data collection and processing of WT and mutant NEC coats in this study.

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    PLOS Pathogens

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    viral proteins ul31ray crystallography showedotherwise deleterious effectscryogenic electron tomographynec mutants designednuclear egress complexinvestigation using xdiv >< pweakened hexagonal latticesuppressor mutation promotedhexagonal lattice reminiscentnuclear egresssuppressor mutationhexagonal latticenec latticehexagonal membraneuniversal suppressorbound latticealternative latticenec oligomerizationnec hexamersnec deformsnec ).vitro stabilization strategypowerful enoughperinuclear spacenew contactsmembrane aroundlatter budsinitial stepdifferent mechanismsbroad range

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