ppat.1010138.s001.docx (1003.6 kB)
Comparison of ALIX and GFP-TSG101 recruitment to the PVM.
journal contribution
posted on 2021-12-13, 18:36 authored by Yolanda Rivera-Cuevas, Joshua Mayoral, Manlio Di Cristina, Anna-Lisa E. Lawrence, Einar B. Olafsson, Romir K. Patel, Dishari Thornhill, Benjamin S. Waldman, Akira Ono, Jonathan Z. Sexton, Sebastian Lourido, Louis M. Weiss, Vern B. CarruthersA. Representative images for segmentation and quantification of GFP-TSG101 and ALIX to the PVM between WT, RΔgra14 and R:GRA14OE. The PV was labeled using an antibody against TgGRA1. Scale bar is 5 μm.
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master manipulator capablelate domain motifsfusogenic parasitophorous vacuolecontains motifs homologousparasite proteins includinghost cytosolic proteinsdense granule proteinsdeficient parasites revealedhost escrt componentsparasite gains resourcestoxoplasma gondii dependent vlp buddinghost escrt machineryparasite uptakehost cytosolhost cellwt parasitesvesicular buddinggondii dense granulesmediate escrtresiding withinreplicative nicherelease assaypvm ).molecular underpinningsmarked reductionlimiting membranelike particlekey pathwayintracellular subsistenceeffectively siphoningcontaining portionalso showaccesses contents1 virus1 gag
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