pcbi.1009132.s010.docx (13.85 kB)
Cignal Reporter Assay used to measure pathway activity and their associated hallmark pathways.
journal contribution
posted on 2021-07-02, 17:35 authored by Alexis M. Thornton, Lishan Fang, April Lo, Maria McSharry, David Haan, Casey O’Brien, Alice H. Berger, Marios Giannakis, Angela N. Brooks(DOCX)
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RASRNF 43 R 117fs RNF 43 G 659fsKRASeVIP 2 softwareAdditional eVIP Pathways analysisexpression-based variant impact phe...Western blot analysischange-of-function mutationG 659fs variantsRNF 43 WTGOFeVIP 2 methodGFP control cDNAgene variantsRNF 43 WT functiongene variant functiondecision tree-based algorithmeVIP 2RNF 43 G 659fsHEK 293T cellsuse gene expression datapathwaycolon adenocarcinoma patient samplesTNFBRAF missense mutationsgene expression signatures
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