pntd.0011745.s005.docx (14.59 kB)
Characteristics of filter paper samples of 314 further analyzed cases.
journal contribution
posted on 2023-11-29, 18:25 authored by Jacob M. Bezemer, Joanna Merckx, Byron P. Freire Paspuel, Manuel Calvopiña, Henry J. C. de Vries, Henk D. F. H. Schallig, Mariska M. G. Leeflang, Nandini DendukuriqPCR: quantitative Polymerase Chain Reaction, hTNF: human Tumor Necrosis Factor, Ct: Cycle Threshold, IQR: Interquartile Range. a hTNF was applied as internal control for sample taking and DNA extraction in a duplex qPCR together with Leishmania rDNA. Ct values have a logarithmic relationship with DNA concentrations and lower Cts indicate higher DNA copy numbers. b Leishmania species was determined in Leishmania 18SrDNA qPCR positive samples by sequencing a gen fragment that codes for the Leishmania Cytochrome B enzyme.
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alone test seemsdiagnostic yield dueestimate test sensitivitycutaneous lesion suspecteddiagnostic accuracy analysisprevalence 88 %)xlink "> clinicalxlink ">diagnostic accuracyobserved prevalenceestimated sensitivityclinical characteristics86 %)83 %)82 %)77 %)66 %)65 %)58 %)study comparedrural ecuadorpredictive valuesparticipants presentinglr ),likelihood ratioslaboratory diagnosisjune 2021january 2019filter paperdna extractedcutaneous leishmaniasiscri 65cri 5cri 49cri 4cri 36cri 3cri 19cri ).credible intervals63 %).(+ 26
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