posted on 2023-11-09, 13:07authored byJingwen Wu, Peilei Wang, Yijia Yin, Jie Liang, Yujiang Fan, Xingdong Zhang, Xianglong Han, Yong Sun
Periodontal bone defect is a common
but longstanding
healthcare
issue since traditional bone grafts have limited functionalities in
regulating complex intraoral microenvironments. Here, a porous cationic
biopolymeric scaffold (CSC-g-nHAp) with microenvironment
self-regulating ability was synthesized by chitosan-catechol chelating
the Ca2+ of nanohydroxyapatite and bonding type I collagen.
Chitosan-catechol’s inherent antibacterial and antioxidant
abilities endowed this scaffold with desirable abilities to eliminate
periodontal pathogen infection and maintain homeostatic balances between
free radical generation and elimination. Meanwhile, this scaffold
promoted rat bone marrow stromal cells’ osteogenic differentiation
and achieved significant ectopic mineralization after 4 weeks of subcutaneous
implantation in nude mice. Moreover, after 8 weeks of implantation
in the rat critical-sized periodontal bone defect model, CSC-g-nHAp conferred 5.5-fold greater alveolar bone regeneration
than the untreated group. This cationic biopolymeric scaffold could
regulate the local microenvironment through the synergistic effects
of its antibacterial, antioxidant, and osteoconductive activities
to promote solid periodontal bone regeneration.