Calibration curve ROS quantification.

Malondialdehyde bis(dimethyl acetal) (MDA) concentration is used as a proxy for ROS accumulation: the higher the MDA concentration, the higher is the ROS abundance in the sample [45]. We chose 6 increasing concentrations of MDA (0, 2.5, 5, 10, 20, 40, 80, 160 μm) and we used 3 replicates per concentration to build a calibration curve. We performed the TBARS assay on each calibration sample as described in [45] and measured the 532 nm absorbance. We subtracted the absorbance of the blank (0 μm MDA) from each calibration sample and plotted MDA concentration versus blank-subtracted absorbance. We obtained a calibration curve and used its parameters to calculate the MDA concentration of the experimental samples shown in Fig 3. The data underlying this figure can be found at https://zenodo.org/records/8033845.

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