Brood size and heat shock survival for _pmiR-238::miR-239a and _pmiR-238::miR-239b.

(A) Results from brood size analysis; miR-238(n4112) (aqua), _pmiR-238::miR-239a (coral), and _pmiR-238::miR-239b (blue) do not have a statistically significant difference when compared to WT (black). ANOVA and the post hoc test (Tukey’s HSD). Bar graph represents mean of three biological replicates; individual replicate data indicated with black dots. The error bars represent SDs. (B) Results from heat shock on day 2 adults for 15 hours at 32°C followed by recovery for 24hr at 20°C. miR-238 (n4112) (aqua), _pmiR-238::miR-239a (coral), and _pmiR-238::miR-239b (blue) do not have a statistically significant different percent heat shock survival when compared to WT (black). ANOVA and the post hoc test (Tukey’s HSD). Bar graph represents mean of three biological replicates; individual replicate data indicated with black dots. The error bars represent SDs.

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