posted on 2021-07-13, 21:34authored byJinyoung Yun, Yeonsu Jeong, Onyou Nam, Ki Baek Yeo, Yun Kee Jo, Hye Ryoung Heo, Chang Sup Kim, Kye Il Joo, Seung Pil Pack, EonSeon Jin, Hyung Joon Cha
Bone
graft materials have been mainly developed based on inorganic
materials, including calcium phosphate. However, these graft materials
usually act as osteoconductive rather than osteoinductive scaffolds.
To improve bone reconstruction, a combination of several materials
has been proposed. However, there are still no alternatives that can
completely replace the existing animal-derived bone graft materials.
In this work, a marine-inspired biomineral complex was suggested as
a potential bone graft material. The proposed biosilicified coccolithophore-derived
coccoliths using bioengineered mussel adhesive proteins show osteopromotive
ability through the synergistic effects of osteoconductivity from
calcium carbonate and osteoinductivity from silica. Its possibility
of use as a bone substitute was determined by evaluating the in vitro
osteogenic behaviors of multipotent mesenchymal stem cells and in
vivo bone regeneration in a rat calvarial defect model. Therefore,
the marine-inspired biomineral complex developed in this study could
be successfully used for bone tissue engineering.