posted on 2021-10-18, 20:14authored byJiawei Guo, Fengwei Li, Fangyuan Cheng, Li Ma, Xiaohui Liu, Pradeepraj Durairaj, Gang Zhang, Dandan Tang, Xiangtian Long, Wei Zhang, Lei Du, Xingwang Zhang, Shengying Li
Human drug metabolites (HDMs) are
important chemicals widely used
in drug-related studies. However, acquiring these enzyme-derived and
regio-/stereo-selectively modified compounds through chemical approaches
is complicated. PikC is a biosynthetic P450 enzyme involved in pikromycin
biosynthesis from the bacterium Streptomyces venezuelae. Here, we identify the mutant PikCD50N as a potential
biocatalyst, with a broad substrate scope, diversified product profile,
and high catalytic efficiency, for preparation of HDMs. Remarkably,
PikCD50N can mediate the drug-metabolizing reactions using
the low-cost H2O2 as a direct electron and oxygen
donor.