posted on 2021-04-13, 12:07authored byXi Feng, Weng-Hang Leong, Kangwei Xia, Chu-Feng Liu, Gang-Qin Liu, Torsten Rendler, Joerg Wrachtrup, Ren-Bao Liu, Quan Li
Correlated
translation-orientation tracking of single particles
can provide important information for understanding the dynamics of
live systems and their interaction with the probes. However, full
six-dimensional (6D) motion tracking has yet to be achieved. Here,
we developed synchronized 3D translation and 3D rotation tracking
of single diamond particles based on nitrogen-vacancy center sensing.
We first performed 6D tracking of diamond particles attached to a
giant plasma membrane vesicle to demonstrate the method. Quantitative
analysis of diamond particles’ motion allowed elimination of
the geometric effect and revealed the net rotation on the vesicle.
6D tracking was then applied to measure live cell dynamics. Motion
characteristics of nanodiamonds on cell membranes under various controlled
physiological conditions suggest that the nanodiamonds’ rotation
is associated with cell metabolic activities. Our technique extends
the toolbox of single particle tracking and provides a unique solution
to problems where correlated analysis of translation and rotation
is critical.