posted on 2022-01-03, 09:29authored byBanu Iyisan, Johanna Simon, Yuri Avlasevich, Stanislav Baluschev, Volker Mailaender, Katharina Landfester
Development of safer
nanomedicines for drug delivery applications
requires immense efforts to improve clinical outcomes. Targeting a
specific cell, biocompatibility and biodegradability are vital properties
of a nanoparticle to fulfill the safety criteria in medical applications.
Herein, we fabricate antibody-functionalized carnauba wax nanoparticles
encapsulated a hydrophobic drug mimetic, which is potentially interesting
for clinical use due to the inert and nontoxic properties of natural
waxes. The nanoparticles are synthesized applying miniemulsion methods
by solidifying molten wax droplets and further evaporating the solvent
from the dispersion. The pH-selective adsorption of antibodies (IgG1,
immunoglobulin G1, and CD340, an antihuman HER2 antibody) onto the
nanoparticle surface is performed for practical and effective functionalization,
which assists to overcome the complexity in chemical modification
of carnauba wax. The adsorption behavior of the antibodies is studied
using isothermal titration calorimetry (ITC), which gives thermodynamic
parameters including the enthalpy, association constant, and stoichiometry
of the functionalization process. Both antibodies exhibit strong binding
at pH 2.7. The CD340-decorated wax nanoparticles show specific cell
interaction toward BT474 breast cancer cells and retain the targeting
function even after 6 months of storage period.