Anti-Inflammatory
Artificial Extracellular Vesicles
with Notable Inhibition of Particulate Matter-Induced Skin Inflammation
and Barrier Function Impairment
posted on 2023-11-20, 17:01authored bySimon Park, Jaesung Lim, Seulgi Kim, Minha Jeon, Hwira Baek, Wooram Park, Juwon Park, Se−Na Kim, Nae-Gyu Kang, Chun Gwon Park, Jin Woong Kim
Particulate
matter (PM) exposure disrupts the skin barrier, causing
cutaneous inflammation that may eventually contribute to the development
of various skin diseases. Herein, we introduce anti-inflammatory artificial
extracellular vesicles (AEVs) fabricated through cell extrusion using
the biosurfactant PEGylated mannosylerythritol lipid (P-MEL), hereafter
named AEVP‑MEL. The P-MEL has anti-inflammatory
abilities with demonstrated efficacy in inhibiting the secretion of
pro-inflammatory mediators. Mechanistically, AEVP‑MEL enhanced anti-inflammatory response by inhibiting the mitogen-activated
protein kinase (MAPK) pathway and decreasing the release of inflammatory
mediators such as reactive oxygen species (ROS), cyclooxygenase-2
(COX-2), and pro-inflammatory cytokines in human keratinocytes. Moreover,
AEVP‑MEL promoted increased expression levels of
skin barrier proteins (e.g., involucrin, IVL) and
water–proteins (e.g., aquaporin 3, AQP3).
In vivo studies revealed that repeated PM exposure to intact skin
resulted in cutaneous inflammatory responses, including increased
skin thickness (hyperkeratosis) and mast cell infiltration. Importantly,
our data showed that the AEVP‑MEL treatment significantly
restored immune homeostasis in the skin affected by PM-induced inflammation
and enhanced the intrinsic skin barrier function. This study highlights
the potential of the AEVP‑MEL in promoting skin
health against PM exposure and its promising implications for the
prevention and treatment of PM-related skin disorders.