pgen.1009805.s011.pdf (111.84 kB)
Alignment of the endogenous S. cerevisiae MUD1 gene to the library’s MUD1 based background gene.
journal contribution
posted on 2021-09-27, 17:40 authored by Dvir Schirman, Zohar Yakhini, Yitzhak Pilpel, Orna Dahan(PDF)
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three functional siteswhose splicing machineriesspecific splicing factorfacilitate alternative splicingeukaryotic gene expressionalternative rna isoformstwo introns mightsplicing machinery tendsmrna expression levelshence efficient splicingdiv >< pgene expressionalternative splicingsplicing machineryrna splicingcryptic splicingmrna moleculemature mrnasplicing regulationefficient regulationvarious speciesvarious extentsvaluable resourcetendency createdsynthetic intronssynthetic fusionselective pressureoriginal siteorganism ’observed tendencylarge librarykey processeventually producecontaining genescombinatorial designbroad analysisbranch siteallow cells>, demonstrating000 variants
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