ppat.1012050.s003.pdf (408.89 kB)

Alamar blue assay of trifluoperazine.

journal contribution

posted on 2024-02-29, 18:55 authored by Sydney Stanley, Xin Wang, Qingyun Liu, Young Yon Kwon, Abigail M. Frey, Nathan D. Hicks, Andrew J. Vickers, Sheng Hui, Sarah M. Fortune

P-value indicates the results of two-way ANOVA with Dunnett’s multiple test correction. Triplicate replicates shown, error bars represent the standard deviation.

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providing experimental evidence div >< p central carbon metabolism 1 virulence system mycobacterium tuberculosis discernible growth defect dependent antibiotic susceptibility mutations functionally increase human host environment global clinical isolates lactate dehydrogenase reveals assessing mtb genes 13 clinical isolates vivo mtb growth lactate dehydrogenase intracellular environment host pressure genes involved dependent l variation across transcriptionally sensitive sugar phosphates strongest targets stepwise nature selective advantage salient feature robust incorporation provides context pressures facing poorly characterized pleiotropic effects mycobacterial cell multifunctional role mtb genome lldd2 lactate accumulation functional reporter detoxification benefit deleterious buildup defining mutations data illustrate bacterial determinants bacterial adaption ancestral lineage

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