13287_2016_467_MOESM8_ESM.doc (37 kB)
Additional file 8: Table S4. of GMP-conformant on-site manufacturing of a CD133+ stem cell product for cardiovascular regeneration
journal contribution
posted on 2017-02-10, 05:00 authored by Anna Skorska, Paula Müller, Ralf Gaebel, Jana Große, Heiko Lemcke, Cornelia Lux, Manuela Bastian, Frauke Hausburg, Nicole Zarniko, Sandra Bubritzki, Ulrike Ruch, Gudrun Tiedemann, Robert David, Gustav SteinhoffStemness marker expression of target and non-target cell fractions presented in the automatically generated cell product (CP). For phenotype characterization the expression of stemness markers (CD117, CD184, CD309, CD14) was evaluated in target cells (CD45+CD34+CD133+) (a) and non-target cells (CD45+CD34−CD133−, CD45+CD34lowCD133−, CD45+CD34+CD133−) (b) using flow cytometry. The respective analysis was made in accordance with ISHAGE guidelines. Data are presented as a mean ± SEM, n = 9. (DOC 37 kb)
