Additional file 6 of Targeting sphingolipid metabolism with the sphingosine kinase inhibitor SKI-II overcomes hypoxia-induced chemotherapy resistance in glioblastoma cells: effects on cell death, self-renewal, and invasion

Additional file 6. Full-length blots of cleaved caspase-3 and GAPDH detection shown in Fig. 3 A. After the protein transfer, the nitrocellulose membrane was cut below the 30 kDa marker, and each strip was incubated with antibodies against GAPDH or Cleaved Caspase-3. Left panel (21 % O2): exposure times were 2 min for GAPDH, 5 min for Cleaved Caspase-3. Right panel (3% O2): exposure times were 2 min for GAPDH, 5 min for Cleaved Caspase-3.