Additional file 5 of Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Additional file 5: Figure S4. Colorimetric read-out of SARS-CoV-2 RT-LAMP. Positive samples 1–5 and Negative samples 1–5 were pre-heated at 90 °C for 5 min before being directly pipetted into RT-LAMP for SARS-CoV-2 targeting ORF8 and N. As internal control (IC) RNase P was additionally targeted. WarmStart Colorimetric LAMP 2x Master Mix (#M1800S, NEB, Ipswich, USA) was used by the manufacturer’s instructions with 5 µL of heated swab sample. For non-template control (NTC) PCR grade water substituted swab sample. Pink colour indicates negative result, yellow signals positive test result.