13046_2020_1740_MOESM4_ESM.pdf (1.71 MB)
Additional file 4 of Unveiling role of sphingosine-1-phosphate receptor 2 as a brake of epithelial stem cell proliferation and a tumor suppressor in colorectal cancer
journal contribution
posted on 2021-04-16, 10:49 authored by Luciana Petti, Giulia Rizzo, Federica Rubbino, Sudharshan Elangovan, Piergiuseppe Colombo, Silvia Restelli, Andrea Piontini, Vincenzo Arena, Michele Carvello, Barbara Romano, Tommaso Cavalleri, Achille Anselmo, Federica Ungaro, Silvia D’Alessio, Antonino Spinelli, Sanja Stifter, Fabio Grizzi, Alessandro Sgambato, Silvio Danese, Luigi Laghi, Alberto Malesci, Stefania VetranoAdditional file 4: Supplementary Figure 4. Gene expression analysis in colon-rectal cancer cell lines and in S1PR2-overexpressing RKO cells. (A) S1PR2 mRNA expression levels in the indicated CRC cell lines. (B) Representative images of migratory and invasive cells (magnification, 4x) are shown. Cell migration and invasiveness were reported as the number of cells in 40 fields of view. (C) Relative expression of metalloproteinases 1–2 (MMP1–2) in S1PR2-overexpressing RKO and scramble cells by qRT-PCR (2 -∆∆Ct method). Data are shown as means ± SEM and are representative of 2 independent experiments, each constituted by three replicates. (D) Ex vivo macroscopic examination of organs (liver, adipose tissue, mesenteric lymph nodes (MLN) and xenograft tumor) in nude mice injected with S1PR2-overexpressing and scramble cells by an imaging system (IVIS) using GFP labeled cells. Data are representative of 2 independent experiments; n = 4.
