13287_2021_2315_MOESM4_ESM.pdf (38.72 MB)
Additional file 4 of 3D-printed nerve guidance conduits multi-functionalized with canine multipotent mesenchymal stromal cells promote neuroregeneration after sciatic nerve injury in rats
journal contribution
posted on 2021-05-30, 03:24 authored by Diego Noé Rodríguez-Sánchez, Giovana Boff Araujo Pinto, Luciana Politti Cartarozzi, Alexandre Leite Rodrigues de Oliveira, Ana Livia Carvalho Bovolato, Marcio de Carvalho, Jorge Vicente Lopes da Silva, Janaina de Andréa Dernowsek, Marjorie Golim, Benedito Barraviera, Rui Seabra Ferreira, Elenice Deffune, Mathues Bertanha, Rogério Martins AmorimAdditional file 4: Figure S1. Immunophenotyping and differentiation potential of canine AdMSCs. (a) Adipogenic differentiation at 14 days. Control of adipogenic differentiation (inserted in a). (b) Osteogenic differentiation at 21 days. Control of osteogenic differentiation (inserted in b). (c) Chondrogenic differentiation at 21 days. Cell micromass after differentiation. (inserted in c). (d) Phenotypic characterization. Gate in canine AdMSCs (cell size versus granularity). (e-h) histograms (negative control - blue; percentage of expression of surface markers - green). (e) CD90: 94,03%; (f) CD45: 2,10%; (g) CD71: 4,71%; (h) CD34: 0,58%. Scale bar, 50 μm.
