Additional file 3 of p38-TFEB pathways promote microglia activation through inhibiting CMA-mediated NLRP3 degradation in Parkinson's disease

Additional file 3: Fig S3. (A, B) Lysates from SNpc of 9 months α-synuclein A53T-tg or wild-type mice were subjected to subcellular fractionation, the nuclear and cytosolic fractions were immunoblotted using the indicated antibodies to determine the levels of TFEB. Data are shown in C. Mean ± SEM, n = 3. *p < 0.05. (C, D) BV2 cells were labeled with Lysosome tracker and visualized lysosome biogenesis under a microscope demonstrating SB203580 increased the levels of lysosome biogenesis and shown in E. Mean ± SEM, n = 10, *p < 0.05. (E) EM images of the lysosomal morphology were shown after SB203580 treatment. (F) The autophagy-related proteins LC3 and P62 were detected in SNpc of brain tissue. (G)Levels of IL-1β and IL-18 were assessed by ELISA. Data were performed using the Student’s unpaired t-test. (H, I) Cell lysates from BV2 cells were immunoblotted to detect the levels of pSer211TFEB and statistically analyzed in M. Mean ± SEM, n = 3.