13024_2021_473_MOESM3_ESM.pdf (2.08 MB)
Additional file 3 of Plaque-associated human microglia accumulate lipid droplets in a chimeric model of Alzheimer’s disease
journal contribution
posted on 2021-07-24, 04:03 authored by Christel Claes, Emma Pascal Danhash, Jonathan Hasselmann, Jean Paul Chadarevian, Sepideh Kiani Shabestari, Whitney E. England, Tau En Lim, Jorge Luis Silva Hidalgo, Robert C. Spitale, Hayk Davtyan, Mathew Blurton-JonesAdditional file 3. TREM2-R47H mutant xMGs reveal no significant increase in the levels of P2RY12, but a decreased trend in the expression of HLA-DR compared to TREM2-WT xMGs in 5X-hCSF1. (A) Quantification of HLA-DR and P2RY12 in GFP TREM2-R47H vs. GFP TREM2 xMGs (green, GFP; red, HLA-DR; purple, P2RY12) surrounding amyloid plaques (blue, Amylo-glo) in 7-month old 5X-hCSF1 reveal (B) a trend towards decrease in total HLA-DR area (P=0.0789) (C) with no significant differences in total plaque area, (D) a trend towards decrease in the percentage of HLA-DR area normalized to plaque area (P=0.1716), and (E) no significant increase in total P2RY12 area (P=0.2848) and (F) percentage of P2RY12 area normalized to plaque area (P=0.3202) in TREM2-R47H vs. TREM2 xMGs. Scale Bar at 10 mm; n=3-4 mice per genotype; 3 images per mouse.
