Additional file 2 of p38-TFEB pathways promote microglia activation through inhibiting CMA-mediated NLRP3 degradation in Parkinson's disease

Additional file 2: Fig S2 Lysates from the cortex and SNpc of mice were immunoblotted using the indicated antibodies. The protein levels of p-p65 were statistically analyzed in B. Mean ± SEM, n = 6, *p < 0.05 (Student’s t-test). (C, D) Cell lysates from BV2 cells were immunoblotted demonstrating SB203580 decreased the levels of NLRP3, ASC, cleaved CASP1 and shown in D. Mean ± SEM, n = 6, *p < 0.05. (E, F) Levels of caspase-1 and IL-1β in tissue homogenates of wild type, α-synuclein A53T-tg and α-synuclein A53T-tg treated with SB203580 were assessed by ELISA assay. (G) The level of mRNA was detected by qPCR. (H) The mouse NLRP3 contains two noncanonical KFERQ-like pentapeptide (355LEKLQ359, 603QIRLE607, 795QKLVE799 and 989EVLKQ993). (I-K) Cell lysates from primary microglia were immunoblotted to detect the LAMP2A and NLRP3 after starvation and analyzed in J and K. Mean ± SEM, n = 3, *p < 0.05. (L, M) Cell lysates from primary microglia were immunoblotted to detect the levels of LAMP2A and NLRP3 after treatment with AR7 and QX77 and statistically analyzed in M. Mean ± SEM, n = 3, *p < 0.05.