Additional file 2 of Whole microbial community viability is not quantitatively reflected by propidium monoazide sequencing approach

Additional file 1: Figure S1. Determining PMA working concentration in E. coli and S. sanguinis cultures. Figure S2. Determining microbial biomass of community samples relative to different concentrations of E. coli cultures. Figure S3. Taxonomic composition of control samples (n=11). Figure S4. Relative abundance of Porphyromonadaceae family with and without PMA treatment in subway samples. Figure S5. Summary of the 606 samples from four datasets used for comparative analysis. Figure S6. Taxa most affected by PMA treatment samples in previous studies compared to the office built environment, saliva, and Boston subway. Figure S7. Taxa least affected by PMA treatment in previous studies compared to the office built environment, saliva, and Boston subway. Figure S8. Approval from the MBTA. Figure S9. Evidence that the spike-in portion of E. coli is likely very similar in sequence to those strains used during the synthetic experiment.