Additional file 2 of Unveiling role of sphingosine-1-phosphate receptor 2 as a brake of epithelial stem cell proliferation and a tumor suppressor in colorectal cancer

Additional file 2: Supplementary Figure 2. Clinical parameters and inflammation score in S1PR2−/− and S1PR2+/+ mice after DSS-induced colitis. Acute colitis was induced in S1PR2−/− and S1PR2+/+ mice by adding filtered 3% DSS to drinking water for 9 days. Representative graphs showing (A) the changes in body weight and (B) disease activity index (DAI) during the entire experiment. (C) The inflammatory status of the colonic mucosa was evaluated accordingly to the Rachmilewitz score by histological analysis. Data are presented as the mean ± SEM, and significance was evaluated by the Mann-Whitney test. Data is representative of 3 experiments (n = 5). (D) Representative histological images of CD45 cells of the colonic mucosa by immunohistochemistry of S1PR2−/− and S1PR2+/+ mice after AOM/DSS-induced colitis-associated cancer. The images were acquired by the DotSlide system at 20x objective. The quantification of CD45 is reported as the CD45 immunoreactive area/40X field. (E) Serum levels of IL-6, IFNy, and IL-17A in S1PR2−/− and S1PR2+/+ mice after AOM/DSS-induced colitis-associated cancer. Data are presented as the mean ± SEM, and significance was evaluated by the Mann-Whitney test. Data is representative of 2 experiments (n = 6). (F) Low-grade adenoma (LGA) in S1PR2−/− and S1PR2+/+ mice after AOM/DSS induced colitis. S1PR2−/− (n = 12) and S1PR2+/+ (n = 10) mice. Means ± SEM.