13068_2021_2079_MOESM2_ESM.pdf (5.41 MB)
Additional file 2 of QTL mapping of a Brazilian bioethanol strain links the cell wall protein-encoding gene GAS1 to low pH tolerance in S. cerevisiae
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posted on 2021-12-17, 04:26 authored by Alessandro L. V. Coradini, Fellipe da Silveira Bezerra de Mello, Monique Furlan, Carla Maneira, Marcelo F. Carazzolle, Gonçalo Amarante Guimaraes Pereira, Gleidson Silva TeixeiraAdditional file 2. Spotting assay for growing at low pH (2.1) of ACY503/CEN.PK113-1A segregants belonging to the “high resistant pool” (A) and “low resistant pool” (B). Each lane represents a different isolated colony and the columns the dilution factor applied to the initial cultures before spotting.
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Fundação de Amparo à Pesquisa do Estado de São Paulo
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regarding bioethanol productionmany biotechnological processesdifferent harsh conditionsbulk segregant analysisgas1 sequence alignmentimprove cell viabilitycell wall proteinmajor qtl associatedindustrial strain pedracerevisiae strains pointedlow ph resistancegas1 allele swapresults collectively showoften impact productivityimprove acid resistancecomplex genetic architecturework revealed gas1encoding gene gas1low ph tolerancelow phallele gas1genetic architecturecell exposuresusceptible strainqtl mappingtolerant strainslaboratory strainsgenetic basisyeast cellsusually challengedtype isolatestraditional foodthroughput approachsynonymous mutationrelevant traitsproductivity losspowerful foundationmodern biofuelmainly duelargely appliedlarge numbergeneration ethanolfermentation standfermentation processevaluated phenotypechromosome xcasual variantbiochemicals factoriesbeverage industriesbased highacidic toleranceacidic environments
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