posted on 2021-08-12, 04:00authored byMelanie Barth, Mehtap Bacioglu, Niklas Schwarz, Renata Novotny, Janine Brandes, Marc Welzer, Sonia Mazzitelli, Lisa M. Häsler, Manuel Schweighauser, Thomas V. Wuttke, Deborah Kronenberg-Versteeg, Karina Fog, Malene Ambjørn, Ania Alik, Ronald Melki, Philipp J. Kahle, Derya R. Shimshek, Henner Koch, Mathias Jucker, Gaye Tanriöver
Additional file 2: Supplementary Fig. 2 Induction of αS aggregation is dependent on endogenous αS expression. Immunofluorescence staining for pS129 showed inclusions in Thy1-h[A53T]αS tg HSCs seeded with Thy1-h[A53T]αS tg brain homogenate. In contrast, there was no induction of αS aggregation in Snca−/− HSCs after seeding using the same brain homogenate as seed. Analysis was done 5 weeks after treatment. See Fig. 1 for methodological detail. The experiment was done twice with 3 cultures each. Scale bar = 500 μm.
Funding
Innovative Medicines Initiative Hertie Institute for Clinical Brain Research (8868)