13046_2021_2176_MOESM2_ESM.pdf (300.96 kB)
Additional file 2 of Hydroxychloroquine synergizes with the PI3K inhibitor BKM120 to exhibit antitumor efficacy independent of autophagy
journal contribution
posted on 2021-11-30, 05:10 authored by Xin Peng, Shaolu Zhang, Wenhui Jiao, Zhenxing Zhong, Yuqi Yang, Francois X. Claret, Moshe Elkabets, Feng Wang, Ran Wang, Yuxu Zhong, Zhe-Sheng Chen, Dexin KongAdditional file 2: Figure S2. HCQ increased the sensitivity of the other PI3K inhibitors in MKN-1 cells. (A) Western blot analysis of LC3B and p62 in MKN-1 cells treated with BKM120 and/or HCQ for 48 h. (B) Cell viability was measured by PrestoBlue after treatment with BAY 80-6946/GDC-0941 alone or in combination with 20 μM HCQ for 72 h. (C) Colony-forming abilities of cells treated with HCQ and/or BAY 80-6946/GDC-0941 were determined. Colony quantification is graphed in (D). (E) Western blot analysis of PI3K-p110α, p-Akt, Akt and p-mTOR after siRNA knockdown of PI3KCA. (F) The cells were treated with HCQ for 48 h in the presence or absence of PI3KCA siRNA, and cell apoptosis was measured. Graphs are normalized to 100% per treatment and shown as mean ± SD from three independent experiments; *p < 0.05; **p < 0.01.
