Additional file 1 of p38-TFEB pathways promote microglia activation through inhibiting CMA-mediated NLRP3 degradation in Parkinson's disease

Additional file 1: Fig S1. (A) Immunohistochemistry (IHC) demonstrating increased α-synuclein in the cortex and SNpc of 9 months α-synuclein A53T-tg or wild-type mice. Statistical analysis of the average score of α-synuclein staining between α-synuclein A53T-tg and wild-type mice. *p<0.05 (Student’s t-test). (B) Immunohistochemistry (IHC) demonstrating increased IBA-1 in the cortex and SNpc of 9 months α-synuclein A53T-tg or wild-type mice. (C) The co-staining between Iba1 and NLRP3 was detected by IF, which the dynamic changes of NLRP3 inflammasome activation in microglia from 3 months to 9 months of age. (D, E) Levels of IL-1β and IL-18 in serum were assessed by ELISA assay in 10 patients suffering from PD and 10 control. Data were performed using the Student’s unpaired t-test.