13059_2021_2390_MOESM1_ESM.pdf (4.56 MB)
Additional file 1 of Transcription shapes DNA replication initiation to preserve genome integrity
journal contribution
posted on 2021-06-10, 03:31 authored by Yang Liu, Chen Ai, Tingting Gan, Jinchun Wu, Yongpeng Jiang, Xuhao Liu, Rusen Lu, Ning Gao, Qing Li, Xiong Ji, Jiazhi HuAdditional file 1: Figure S1. Experimental procedure for NAIL-seq. Figure S2. Comparison among NAIL-seq, Repli-seq, OK-seq, SNS-seq, and EdU/HU-seq in K562 or mouse primary splenic B cells. Figure S3. The landscape of ERIZs. Figure S4. Transcription disturbance relocates ERIZs in K562 cells, mSBCs, and mESCs. Figure S5. Transcription inhibition does not lead to ORC2 relocation. Figure S6. dCas9-mediated transcription blockade induces MCM5 accumulation and replication initiation. Figure S7. Collision of transcription and DNA replication initiation induces genome instability. Figure S8. The full uncropped pictures for Western blots.
