Additional file 1 of Transcription-dependent confined diffusion of enzymes within subcellular spaces of the bacterial cytoplasm

Additional file 1: Figure S1. Genetic organization of the rib-operon from B. subtilis and corresponding enzyme catalyzed reactions of RF biosynthesis. Figure S2. Model for heavy RF synthase. Figure S3. In-gel fluorescence detection of fusion enzymes from SDS-PAGE analysis displaying full length fusion enzymes. Figure S4. SPT method used to detect and analyze fusion enzymes in live cells of B. subtilis. Figure S5. Spatial distributions of detected spots for all four Rib FP enzymes in a cell size-dependent manner and localization of the nucleoid(s) in exponentially grown cells. Figure S6. Localization error histograms and cell size boxplots for all SPT datasets in this study. Figure S7. Transition analysis of RibDG-mV in the absence and presence of RibH and dwell-times for different numbers of confined steps. Figure S8. Spots detected per frame and per frame and cell for RibH-mV in the presence and absence of ribE. Figure S9. RibE shows different modes of diffusion in the bacterial cytoplasm. Figure S10. RibAB shows different modes of diffusion in the bacterial cytoplasm. Figure S11. RibDG shows different modes of diffusion in the bacterial cytoplasm. Figure S12. EAMSD plots for trajectories classified as anomalous of all four Rib FP enzymes. Table S1. Summary of confinement analysis from all SPT data used in this study. Table S2. Mean estimated localization errors and radii of confinement for each SPT dataset used in this study. Table S3. List of diffusion coefficients derived from structural data which have been calculated. Table S4. Strains used in this study. Table S5. Antibiotics and drugs used for cultivation of bacteria in this study. Table S6. Plasmids used in this study. Table S7. Oligonucleotides used in this study.