Additional file 1 of The unusual gene architecture of polyubiquitin is created by dual-specific splice sites

Additional file 1: Figure S1. Differences between recursive splice sites (RSSs) and dual-specific splice sites (DSSs). Figure S2. Dual-specific splice sites support splicing activity with weaker substrate sequences. Figure S3. The effect of overexpressing HA-tagged ubiquitin in HEK293 cells. Figure S4. RT-PCR analysis of the splicing pattern of mouse UBC genes from brain, liver, and muscle tissues. Figure S5. Evolution of polyubiquitin gene family. Figure S6. Sequence of insert variant X-6533768-T-TCTCGCTCTCCTGACTCAGTGGTTCCTCCACCTGGCTCTCCTGACTCAGTGGTTCTTCCAC in VCX3A, which matches intron 3 of ENST00000398729. Figure S7. Distribution of splice site sequences of introns whose length is a multiple of a surrounding tandem repeat’s unit length. Table S1. Lariat reads recovered from the 5′ splice sites of unannotated dual splice sites. Table S2. Two isoform sequences in Fig. 4. Table S3. Ubiquitin subunit counts of UBC orthologs. Table S4. Introns in tandem repeats.