12934_2020_1404_MOESM1_ESM.docx (577.63 kB)
Additional file 1 of The length of ribosomal binding site spacer sequence controls the production yield for intracellular and secreted proteins by Bacillus subtilis
journal contribution
posted on 2020-07-30, 06:20 authored by Kristina Volkenborn, Laura Kuschmierz, Nuka Benz, Patrick Lenz, Andreas Knapp, Karl-Erich JaegerAdditional file 1: Fig. S1. Influence of spacer length on the production of target proteins. Fig. S2. Influence of spacer composition on the production of GFPmut3 and SPPel-Cut-11. Fig. S3. In silico analysis of mRNA secondary structures. Table S1. Primers used in this study. Table S2. Changes in transcript amounts of target genes with different spacer lengths.