Additional file 1 of The genome and sex-dependent responses to temperature in the common yellow butterfly, Eurema hecabe

Additional file 1: Figure S1. GenomeScope v2.0 k-mer profile plot based on 21-mers in Illumina reads. The observed k-mer frequency distribution is depicted in blue, whereas the GenomeScope fitmodel is shown as a black line. The unique and putative error k-mer distributions are plotted in yellow and red, respectively. Figure S2. Oxford dot plots of orthologous genes between the different lepidopteran species. Orthologous genes are coloured according to their positions in the reference species at the horizontal axis. Figure S3. Transposable elements. Figure S4. Prcomp principal components and heatmaps of all samples. Figure S5. Differentially expressed gene clusters of different combinations of E. hecabe sexes and tissues under different heat-stress experiment. Genes differentially expressed between different temperatures (18°C, 25°C and 30°C) in different tissues (H: head; B: body) of both sexes (F: female; M: male) were identified from strand-specific RNA-Seq using EdgeR with three biological replicates (each replicate annotated with number 1, 2 and 3) per sample. Figure S6. Differentially expressed protein-coding genes under various temperature settings. Venn diagrams showing the numbers of common and sex-specific differential expressed protein-coding genes when comparing the expression at 18°C with 25°C, 25°C with 30°C and 18°C with 30°C. Figure S7. GO annotation of differentially expressed genes in female Eurema hecabe at 30°C when comparing to 18°C. Figure S8. GO annotation of differentially expressed genes in male Eurema hecabe at 30°C when comparing to 18°C. Figure S9. GO annotation of differentially expressed genes in female Eurema hecabe at 30°C when comparing to 25°C. Figure S10. Differentially expressed microRNAs at different temperatures. Venn diagrams showing the numbers of common and sex-specific differential expressed miRNAs when comparing the expression at 18°C with 25°C and 18°C with 30°C. Figure S11. Neuropeptides in E. hecabe. (A) The neuropeptide genes identified in the E. hecabe genome and their expression in different life stages. (B) Expression of neuropeptide genes under different temperatures. F: female; M, male; H: head; B: body.