Additional file 1 of The CRK2-CYC13 complex functions as an S-phase cyclin-dependent kinase to promote DNA replication in Trypanosoma brucei

Additional file 1: Figure S1. Sequence alignment of the twelve cyclin proteins (CYC2-CYC13) in T. brucei. Figure S2. Co-immunoprecipitation to test the in vivo interaction between CYC13 and CRK3 and between CYC13 and CRK12. Figure S3. Quantification of KKT13 fluorescence intensity in non-induced control, CRK2 RNAi and CYC13 KPP1 RNAi cells. Figure S4. Coverage of the peptides detected by mass spectrometry in purified GST-Sld5 and GST-Mcm3 after in vitro kinase assay with CRK2-CYC13. Figure S5. Effect of epitope tagging of Mcm3 on its function and cell growth. Figure S6. Ectopic overexpression of Mcm3 and its mutant in the 29–13 strain.