Additional file 1 of Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor

Additional file 1: Supplementary figures and tables. Figure S1. FACS analysis to validate the prime-editing system. Figure S2. Optimization of the prime-editing efficiency using various lengths of pegRNAs and proximal dsgRNA at the Igf2 and Adamts20 target sites. Figure S3. Improvement of prime-editing efficiency with proximal dsgRNAs in mouse cell lines. Figure S4. Improvement of prime-editing efficiency with chromatin-modulating peptides in mouse cell lines. Figure S5. Relative fractions of intact genomic DNA from closed chromatin and open chromatin regions in NIH/3T3 and C2C12 cells. Figure S6. Generation of F1 mice via germline transmission from Igf2 mutant mice. Figure S7. Indel frequencies at the potential off-target sites of pegRNA and nsgRNA used for targeted mutagenesis of the Igf2 target site. Table S1. Targeted mutagenesis in mouse embryos. Table S2. Sequences of pegRNAs, nsgRNAs, and dsgRNAs used in this study. Table S3. Sequencies of the on-target and the potential off-target sites of pegRNA and nsgRNA used for targeted mutagenesis of the Igf2 target site. Table S4. Primer sequences used to amplify the target DNA in this study. Table S5. Primer sequences used for real-time qPCR. Table S6. Amino acid sequences of CMP-PE-V1 and CMP-PE-V2.