Additional file 1 of TLR7 is expressed by support cells, but not sensory neurons, in ganglia

Additional file 1: Figure S1. In situ hybridization positive and negative controls for mouse dorsal root and vagal ganglia. Expression of the housekeeping gene piptidylprolyl isomerase B (Ppib), a cyclosporine-binding protein, was detected in serial sections of wild type and TLR7 knockout (KO) mouse (A) dorsal root ganglia and (B) vagal ganglia, confirming that RNA was present all the tissue. A negative control probe for the bacterial gene dihyrdodipicolinate reductase (DapB) was not detected in wild type or TLR7 KO (A) dorsal root ganglia and (B) vagal ganglia, excluding the presence of non-specific probe binding. Scale bar = 50 μm. Blue = hematoxylin nuclear stain.