13046_2021_1909_MOESM1_ESM.pdf (860.26 kB)
Additional file 1 of Small-molecule HDAC and Akt inhibitors suppress tumor growth and enhance immunotherapy in multiple myeloma
journal contribution
posted on 2021-03-25, 08:40 authored by Mitsuhito Hirano, Yoichi Imai, Yuta Kaito, Takahiko Murayama, Kota Sato, Tadao Ishida, Junichi Yamamoto, Takumi Ito, Muneyoshi Futami, Masaki Ri, Hiroshi Yasui, Tamami Denda, Yukihisa Tanaka, Yasunori Ota, Masanori Nojima, Yasuhiko Kamikubo, Noriko Gotoh, Shinsuke Iida, Hiroshi Handa, Arinobu TojoAdditional file 1: Figure S1. HDAC inhibitors upregulate NKG2D ligands. (a–d) MICA and MICB mRNA levels in RPMI8226 and U266 cells exposed to panobinostat/ACY-1215. Experiments were performed in triplicate (n = 3). (*p < 0.05, **p < 0.01, “ns” indicates no significant difference.) (e) The ratio of mRNA expression for MICA and MICB between cells exposed to panobinostat, romidepsin, ACY-1215 and those exposed to DMSO for 0 h and 4 h. (f) IKZF1 mRNA levels in U266 cells exposed to panobinostat. (**p < 0.01) (g-h) Prognostic value of NKG2D ligand expression based on MM patient clinical trial data.
