Additional file 1 of Prostaglandin D2 metabolites activate asthmatic patient-derived type 2 innate lymphoid cells and eosinophils via the DP2 receptor

Additional file 1: Table S1. Detailed information on flow cytometric antibodies used for cell sorting of ILC2s. Table S2. Calculated agonist EC70 values for PGD2 and seven selected PGD2 metabolites. Table S3. Characteristics of study subjects. All subjects had a history of allergic asthma since at least 12 months. BMI had to be between 19 to 32 kg/ m2. Figure S1. Eosinophil shape change induced by PGD2, DK-PGD2, ∆12-PGD2, 15-deoxy-∆12,14-PGD2, PGJ2, 9a,11b-PGF2, ∆12-PGJ2 and 15-deoxy-∆12,14-PGJ2. Granulocytes were isolated from whole blood of asthmatic patients and were incubated with increasing concentrations of metabolites, n=3, 9α,11β-PGF2: n=2. The mean fluorescence values of the forward scatter were determined by flow cytometry and the percentage of shape change above basal was calculated. Values are given as mean ± SD. Figure S2. Concentration of IL-5 cytokine secretion of ILC2s in the presence of ascending concentrations of PGD2 and selected metabolites, n=3. Values are given for three different subjects (circle, square and triangle). Figure S3. Concentration of IL-13 cytokine secretion of ILC2s in the presence of ascending concentrations of PGD2 and selected metabolites, n=3. Values are given for three different subjects (circle, square and triangle).