Additional file 1 of PAR-4/Ca2+-calpain pathway activation stimulates platelet-derived microparticles in hyperglycemic type 2 diabetes

Additional file 1: Figure S1. Correlations between CD62P+ MPs and fasting glucose level in all groups. Statistical significance was determined with linear regression. Dotted lines indicated the 95% of interval confidence. Figure S2. a–c Representative Western blots and densitometric analysis of PAR-1 and PAR-4 protein expression in platelets from NGT, GGC and PGC. The results were expressed relative to the control on the same blot, defined as 100%, and by the protein of interest/β actin densitometric ratio. The p-values were evaluated by ANOVA: PAR-1, p = 0.9; PAR-4, p < 0.0001 followed by a post-hoc Bonferroni test. d Counts of platelets-derived microparticles (PMP) released by platelets from NGT, GGC and PGC treated with AY-NH2, (PAR-4 agonist). The p-values were evaluated by ANOVA: p = 0.006. e Calpain activity in platelets from NGT, GGC and PGC, stimulated with AY-NH2 (200 μM). Calpain activity was determined as the value of luminescence recorded as relative light units (RLU) per µg of protein lysate. The p-values were evaluated by ANOVA: p < 0.0001 followed by a post-hoc Bonferroni test. Values are mean ± SEM.