Additional file 1 of Oral microbiota disorder in GC patients revealed by 2b-RAD-M

Additional file 1: Figure S1. Diversity, abundance, and distribution comparison of bacteria and fungi between gastric cancer (GC) tissues and adjacent normal tissues. Venn diagram showed the shared and unique bacteria(A) and fungi(B) species between GC tissues and adjacent normal tissues. Bacterial(C) and fungal(D) abundance and distribution in the two groups showed by barplot at phylum, genus, and species level. The composition of bacteria(E) and fungi(F) of GC tissues and adjacent normal tissues is shown by heat maps. The bacteria(G) and fungi(H) alpha diversity (Chao1, Shannon index, and Simpson index) of GC tissues and adjacent normal tissues. Figure S2. Comparison of tongue coating bacteria between gastric cancer(GC) patients and healthy controls. A, Shared and unique species between the two groups presented by Venn diagram. B, Comparison of alpha diversity (Chao1, Shannon index, and Simpson index) between the two groups. C, Comparison of beta diversity (Bray–Curtis distance, Binary Jaccard distance and Euclidean distance) between the two groups. D, The relative abundance and distribution of salivary bacteria at phylum, genus, and species level. E, The top 10 species with different abundance between the two groups. Figure S3. Comparison of the relative abundance of salivary(A) and tongue coating(B) bacteria at the phylum level between the two groups. Figure S4. Analysis of salivary bacteria as biomarkers for the diagnosis of gastric cancer (GC). A, Indicator analysis of salivary bacteria between the two groups. B, The mean decrease accuracy of salivary Prevotella melaninogenica was the largest. C, The salivary Prevotella melaninogenica achieved an area under the receiver operating characteristic curve (AUC) of 0.620 for the classification of the GC group from the control group. Figure S5. Analysis of tongue coating bacteria as biomarkers for the diagnosis of gastric cancer (GC). A, Indicator analysis of tongue coating bacteria between the two groups. B, The mean decrease accuracy of tongue coating Prevotella melaninogenica was the largest. C, The tongue coating Prevotella melaninogenica achieved an area under the receiver operating characteristic curve (AUC) of 0.536 for the classification of the GC group from the control group. Figure S6. Comparison of relative abundance of salivary fungi by Analysis of Variance (ANOVA) at phylum(A), genus(B), and species(C) level. Figure S7. Comparison of relative abundance of tongue coating fungi by Analysis of Variance (ANOVA) at phylum(A), genus(B), and species(C) level. Table S1. Demographic and clinical features of patients who provided gastric specimens. Table S2. Adaptors and primers used for 2bRAD-M library preparation.