Additional file 1 of Neddylation-dependent protein degradation is a nexus between synaptic insulin resistance, neuroinflammation and Alzheimer’s disease

Additional file 1. Fig. S1. Induction of synaptic IR in primary neurons. Fig. S2. Proteasomal degradation of IRS2 is not affected by neddylation. Fig. S3. CUL7 KD does not alter insulin signaling and Aβ3(pE) application exacerbates IR. Fig. S4. MetS induces neuronal cell loss, astroglial and microglial activation in the hippocampal CA1 region of heterozygous TBA2.1 mice. Fig. S5. Baseline recordings of fEPSP in hippocampal acute slices from WT and heterozygous TBA2.1 mice fed with a RD or HFD. Fig. S6. Characterization of the effects of MLN-4924 on basic metabolic parameters, basal fEPSP slope and LTD-dependent learning and memory in WT and heterozygous TBA2.1 mice fed with a RD or HFD. Table S1. Expression constructs and antibodies used in this study.