Additional file 1 of Mesenchymal stem cells derived from human induced pluripotent stem cells improve the engraftment of myogenic cells by secreting urokinase-type plasminogen activator receptor (uPAR)

Additional file 1: Figure S1 (related to Fig. 1). No detectable expression of pluripotency factors OCT3/4, NANOG, or SOX2 in iMSCs. Figure S2 (related to Fig. 2). Proliferation of human myogenic cells was stimulated by coculture with iMSCs or BM-MSCs. Figure S3 (related to Fig. 3). Representative immunohistochemistry of cross-sections of the TA muscles of NSG-mdx4Cv mice or NOD/Scid mice transplanted with Hu5/KD3 cells, or Hu5/KD3 cells with iMSCs (i.p.). Figure S4. Grip test and treadmill test of mdx mice and Sirius staining of diaphragm in NSG-mdx4cv mice injected intraperitoneally with iMSCs or BM-MSCs. Figure S5.BM-MSCs or iMSCs did not affect fiber size in TA muscles of NSG-mdx4Cv or mdx mice. Figure S6 (related to Table 1). iMSCs and BM-MSCs secrete similar but distinct sets of cytokines. Figure S7 (related to Figure 4). Phase contrast images of Hu5/KD3 cells just after scratching with a pipette tip (0 h) and 6 h later (6 h).