Additional file 1 of Improving the ameliorative effects of berberine and curcumin combination via dextran-coated bilosomes on non-alcohol fatty liver disease in mice

Additional file 1: Figure S1. Optimization on the LR of LSDBC in terms of (A) external phase pH and (B) incubation time. The LC of LSDBC was optimized in terms of (C) external phase pH and (D) incubation time. Each bar represents the mean ± SD (n = 3). Figure S2. Optimization on the LR of LSDBC in terms of (A) the weight ratio of SPC to CHOL and (B) SPC to BER. The LC of LSDBC was optimized in terms of (A) the weight ratio of SPC to CHOL and (B) SPC to BER. Each bar represents the mean ± SD (n = 3). Figure S3. Transportation of (A) BER and (B) CUR across mucus layer via DEAE-DEX@LSDBC with various amount of DEAE-DEX. Each bar represents the mean ± SD (n = 3). Figure S4. UV spectrum of BER, CUR, LIP, LBC, LSDBC and DEAE-DEX@LSDBC. Figure S5. Cytotoxicity of preparations against Caco-2 cells and LO2 cells. Each bar represents the mean ± SD (n = 3). Figure S6. Synchronized biodistribution of BER and CUR from preparations orally delivery in the male Kunming mice. The T/P ratios for BER and CUR from (A–C) BER+CUR, (D–F) LBC, (G–I) LSDBC and (J–L) DEAE-DEX@LSDBC in the heart, liver, spleen, lung and kidney at (A, D, G, J) 1 h, (B, E, H, K) 6 h and (C, F, I, L) 24 h after orally administration. Each bar represents the mean ± SD (n = 3). *p < 0.05, **p < 0.01 and ***p < 0.001 BER vs CUR in various tissues. Figure S7. Representative morphological images of Oil-Red-O staining on LO2 cells after 12 h incubation with various preparations (Scar bar 50 μm). Figure S8. Drug release behavior of CUR and BER in both SIF and SGF medium for 24 h. Each symbol represents the mean ± SD (n = 3). Table S1. Pharmacokinetics parameters of BER after oral administration (n = 5). Table S2. Pharmacokinetics parameters of CUR after oral administration (n = 5).