Additional file 1 of IDH2/R140Q mutation confers cytokine-independent proliferation of TF-1 cells by activating constitutive STAT3/5 phosphorylation

Additional file 1: Figure 1. TF-1(WT) and TF-1(R140Q) cells were cultured in the absence or presence of 5 ng/mL GM-CSF for 24 h, and PARP and Bcl-xL expression levels were analyzed by western blotting. GAPDH was used as a loading control. Figure 2. TF-1(WT) and TF-1(R140Q) cells were cultured in the absence or presence of 5 ng/mL GM-CSF for 24h and subjected to western blotting for detection of the indicated proteins. β-Actin was used as a loading control. Figure 3. TF-1(R140Q) cells were treated with AGI-6780 for 2 days and subjected to western blotting for detection of the indicated proteins. GAPDH was used as a loading control. Figure 4. TF-1(R140Q) cells were treated with C188-9 or NSC74859 for 24 h and subjected to western blotting for detection of the indicated proteins. β-Actin was used as a loading control.