Additional file 1 of Extracts of Sida cordifolia contain polysaccharides possessing immunomodulatory activity and rosmarinic acid compounds with antibacterial activity

Additional file 1 Table S1. Composition of S. cordifolia aqueous fractions (SCAF). Table S2. Primers sequences (forward and reverse) used for quantification of cytokine mRNA expression in splenocytes and RAW246.7 macrophage cell line. Table S3: MIC and MBC (mg/ml) of Sephadex LH-20 fractions (SCMEXBu) compared to crude methanol extract (MEX). Fig. S1. (A) Typical melt curve for murine inducible nitric oxide synthase (iNOS) (B) Amplification curve of iNOS following incubation of RAW 264.7 cells with SCAF 5 showing untreated control versus treatment with SCAF 5. (C) Amplification for IL-6 following incubation of splenocytes with SCAF 5. Lines show untreated control versus treatment with SCAF 5. Fig. S2. Mass Spectrum of SC2 (A) Mass Fragmentation patterns obtained for SC2 were suggestive of rosmarinic acid. (B) 13C NMR spectra of SC2. (C) Table summarising chemical shifts of both 13C NMR and 1H NMR spectra for SC2. 13C NMR and 1H NMR shifts and proposed structure of SC2 were highly suggestive of rosmarinic acid. (D) The proposed structure of SC2 (rosmarinic acid). Fig. S3. Mass Spectrum of SC1 (A) Mass Fragmentation patterns obtained for SC1 were suggestive of rosmarinic acid 4-O-β-d-glucoside (Rosmarinyl glucoside). (B) 13C NMR spectra of SC1. (C) Table summarising chemical shifts of both 13C NMR, and 1H NMR. 13C NMR, and 1H NMR shifts were highly suggestive of rosmarinic acid 4-O-β-d-glucoside. and the (D) proposed structure of SC1 (rosmarinic acid 4-O-β-d-glucoside).